[amazon_link id=”1574445545″ target=”_blank” ][/amazon_link]Several months ago a second edition of  “[amazon_link id=”1574445545″ target=”_blank” ]Handbook of HPLC[/amazon_link]” was released. The new edition avoids well-established theoretical and practical aspects of HPLC that were extensively covered in the first edition published in 1998. However, the editors greatly update the handbook with emerging novel methods and concepts  that have contributed to […]

In September 2008 almost 52,000 infants in China suffered urinary problems from consumption of melamine contaminated baby-formulas and some other dairy products (WHO, 2009). By the end of November 2008 almost 300,000 infants were affected and six deaths have been confirmed by the Chinese Ministry of Health. A low level of melamine contamination was also found […]

Research scientists from Schering-Plough Research Institute developed a rapid high-performance liquid chromatography (HPLC) technique that allows for efficient separation and analysis of pharmaceutical compounds. The method uses a short column packed with 2.7μm “fused-core” silica particles that are made by fusing a 0.5μm layer of porous silica onto a solid silica particle[1]. These unique particles […]

“HPLC for Pharmaceutical Scientists” is a new hardcover book edited by Yuri V. Kazakevich and Rosario LoBrutto with a price tag of $159 at Amazon. This is an excellent book for both novice and experienced pharmaceutical chemists that provides a unified approach to HPLC with a balanced treatment of the theory and practice of HPLC […]

How many of you out there picked or even ate fresh bilberries? Probably not too many, unless you are from Northern Europe, Russia, or the Rocky Mountains of the US. The rest of us find bilberries in the form of purple capsules in dietary supplements. Bilberry is small shrub to a one foot in height […]

Here is another example of forensic application of HPLC that allows to classify and date the black gel pen ink entries on documents. Gel pens first came out in 1984 in Japan and quickly became popular throughout the world due to their solid line, bold colors, and low cost. They are also regarded as environmentally […]

The HPLC method used a C8 column and the potassium chloride in aqueous acetic acid as a mobile phase with a linear gradient. The retention time was a bit over 20 min and the identity of the peak was confirmed as furosine by MS (mass spectrometry).

During the heating process carbonyl groups of sugars react with amino groups of amino acids and peptides forming a variety of products. The products of this, so-called, Maillard reaction contribute to flavor, aroma, and color of soy milk.

Nano HPLC is a relatively new development in chromatography world driven by recent advancements in proteomics that would require decreasing of inner diameter (ID) of liquid chromatography (LC) column to allow for a smaller sample amount and to increase sensitivity.

Scientist at Weber State University developed methods for extraction, identification and quantitation of xanthones and their derivatives: alpha-mangostin, 8-desoxygartanin, gartanin, beta-mangostin, 3-mangostin, and 9-hydroxycalabaxanthone.