Nano LC Sensitivity

In theory, the analyte sensitivity increases on decreasing the column inner diameter.

Columns in Nano LC

As I mentioned in the last post from this series, capillary columns of 10–100 μm inner diameter are commonly used in nano-liquid chromatography setups. They are typically made either from fused silica or PEEK materials currently found in your everyday Gas Chromatography or HPLC, respectively. There are three basic types of capillary columns used in […]

It is interesting to note that 20 years later there is still no clear definition of nano-LC. Due to a relatively large size of the columns used in nano-HPLC (10-140 μm), the method does not fall within the realm of the conventional definition of nanotechnology – “the control of matter on a scale smaller than […]

Thanks to one of the readers of this blog – Farooq, I now have the full text of an excellent, 21-page review by the researches from Rome and Spain entitled “Recent applications in nanoliquid chromatography” (DOI 10.1002/jssc.200700061). Two months ago, I did a small article about nano liquid chromatography to answer a question posted on […]

A review was published yesterday in “Journal of Separation Science” by a group of Italian researchers. So far all I have is abstract: Since its first introduction by Karlsson and Novotny in 1988 nano-LC has emerged as a complementary and/or competitive separation method to conventional HPLC, offering several advantages such as higher efficiency, ability to […]

Nano Liquid Chromatography

Nano HPLC is a relatively new development in chromatography world driven by recent advancements in proteomics that would require decreasing of inner diameter (ID) of liquid chromatography (LC) column to allow for a smaller sample amount and to increase sensitivity.


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