In theory, the analyte sensitivity increases on decreasing the column inner diameter.

As I mentioned in the last post from this series, capillary columns of 10–100 μm inner diameter are commonly used in nano-liquid chromatography setups. They are typically made either from fused silica or PEEK materials currently found in your everyday Gas Chromatography or HPLC, respectively.

There …

It is interesting to note that 20 years later there is still no clear definition of nano-LC. Due to a relatively large size of the columns used in nano-HPLC (10-140 μm), the method does not fall within the realm of the conventional definition of nanotechnology - “the control of matter …

Thanks to one of the readers of this blog - Farooq, I now have the full text of an excellent, 21-page review by the researches from Rome and Spain entitled “Recent applications in nanoliquid chromatography” (DOI 10.1002/jssc.200700061).

Two months ago, I did a small article about nano …

A review was published yesterday in “Journal of Separation Science” by a group of Italian researchers. So far all I have is abstract:
Since its first introduction by Karlsson and Novotny in 1988 nano-LC has emerged as a complementary and/or competitive separation method to conventional …

Nano HPLC is a relatively new development in chromatography world driven by recent advancements in proteomics that would require decreasing of inner diameter (ID) of liquid chromatography (LC) column to allow for a smaller sample amount and to increase sensitivity.