Comments on: Nano Liquid Chromatography

By: Jennifer Ronson

Jennifer Ronson — Mon, 13 Dec 2010 12:08:46 +0000

Does this technology have any real future? Does it offer opportunities? I am asking because you said that there are only 2 companies currently manufacturing LC chips and I guess there should be more. From the description it seems like a really viable option.

By: Mr.Keyur patel

Mr.Keyur patel — Sat, 09 Jan 2010 14:14:20 +0000

please send me the detailed information of nano-Lc for my poster presentation.

By: Jenny Butler

Jenny Butler — Sun, 01 Nov 2009 22:24:52 +0000

hi i’m researching ‘chromatography on a chip’ for some students and just enquiring about how you refer to nano chromatography does it mean the same thing? Would you also be able to recommend any references on the principles of chromatography on a chip? Thank you kindly.

Regards,
JB

By: Dr Matthews

Dr Matthews — Mon, 13 Oct 2008 04:55:25 +0000

A really cool article that gives information on the fundamentals is Patel & Weber, Electrophoresis in free fluid, J. Biol. Phys. Chem. 3 (2003) 60-73.

By: Power Of Law Forms

Power Of Law Forms — Tue, 06 Nov 2007 07:20:00 +0000

Power Of Law Forms…

I couldn’t understand some parts of this article, but it sounds interesting…

By: Nano Liquid Chromatography - Reloaded | Chromatography

Nano Liquid Chromatography - Reloaded | Chromatography — Sun, 17 Jun 2007 19:08:26 +0000

[...] months ago, I did a small article about nano liquid chromatography to answer a question posted on Yahoo Answers but after reading “Recent applications in [...]

By: Chemist

Chemist — Fri, 08 Jun 2007 19:43:23 +0000

Dr. Imran,

Is that going to be a review book or something else?

By: DR. Imran ali

DR. Imran ali — Fri, 08 Jun 2007 10:16:23 +0000

Nowadays, Im writing a book on nano chromatography. If u have any literature kindly send me.

regards,

By: Chemist

Chemist — Wed, 02 May 2007 14:26:43 +0000

I appreciate your kind comments :) thank you. Check out these articles:

“Variable-gradient generator for micro- and nano-HPLC”

Achille Cappiello, Giorgio Famiglini, Chiara Fiorucci, Filippo Mangani, et al. Analytical Chemistry. Washington: Mar 1, 2003. Vol.75, Iss. 5; pg. 1173

A new, simple device generates accurate nano- and microflow rate gradients from any conventional HPLC system. The core of the new device is represented by an electric-actuated, computer-controlled, multiposition HPLC valve. The valve hosts six reservoirs for as many different mobile-phase compositions of increasing strength. A low flow rate stream pushes the weakest solvent through the column as long as required and at the desired flow rate, until the chromatographic run is started. From this time on, the electric actuation allows one to select which reservoir will be on-line with the column and for how long, thus generating a specific solvent gradient, through a sequence of controlled segments of precise mobile-phase composition. Ibis permits one not only to exactly reproduce the programmed slope but also to achieve different gradient shapes (i.e., linear, convex, concave) for different separation needs. The new device has proven to be reliable and reproducible even at the lowest flow rate tested (250 nL-min-1) and in different chromatographic conditions.

“Scaling and the design of miniaturized chemical-analysis systems”

Nature 442, 374-380 (27 July 2006) | doi:10.1038/nature05059; Published online 26 July 2006

Micrometre-scale analytical devices are more attractive than their macroscale counterparts for various reasons. For example, they use smaller volumes of reagents and are therefore cheaper, quicker and less hazardous to use, and more environmentally appealing. Scaling laws compare the relative performance of a system as the dimensions of the system change, and can predict the operational success of miniaturized chemical separation, reaction and detection devices before they are fabricated. Some devices designed using basic principles of scaling are now commercially available, and opportunities for miniaturizing new and challenging analytical systems continue to arise.

By: farooq

farooq — Wed, 02 May 2007 06:29:56 +0000

Again many many thanks, you are really awsome in providing knowledge to scientific community, well as a matter of fact i have access to almost all journals from almost all publishers because i am a student & doing my research now a days, so if you know any other articles then please give me those. Really bundle of thanks!

By: Chemist

Chemist — Tue, 01 May 2007 20:39:41 +0000

No problem! As far as references go; there is actually a journal “Lab on a Chip” published by RSC (http://www.rsc.org/Publishing/Journals/lc/) dedicated entirely to the subject Miniaturization for Chemistry, Biology and Bioengineering but most articles are not available without a subscription. There is quite a few manufactures that publish information on their websites, it is a hot field these days:

http://www.agilent.com/chem/chip
http://www.coventor.com/microfluidics/labonchip.html

Nano HPLC is basically a subset of “Lab on a Chip” technology, so just google “Lab on a Chip” and then look for LC related sites.

Also these books might be helpful to you:

“HPLC Made to Measure: a practical handbook for optimization”

Lab-on-a-Chip: Miniaturized Systems for (Bio)Chemical Analysis and Synthesis

By: farooq

farooq — Tue, 01 May 2007 09:05:44 +0000

Thanks Chemist for your detailed reply, not only it helps me a lot but it also increases my interest in nano HPLC, Can you please give me some reference web sites or books from where i can get more detailed knowledge related to its instrumentations & construction (& also about microfluidic columns). Once again many many thanks.