Articles on: HPLC

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Several months ago a second edition of  “” was released. The new edition avoids well-established theoretical and practical aspects of HPLC that were extensively covered in the first edition published in 1998. However, the editors greatly update the handbook with emerging novel methods and concepts  that have contributed to the further advancement of HPLC technique in the […]

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In September 2008 almost 52,000 infants in China suffered urinary problems from consumption of melamine contaminated baby-formulas and some other dairy products (WHO, 2009). By the end of November 2008 almost 300,000 infants were affected and six deaths have been confirmed by the Chinese Ministry of Health. A low level of melamine contamination was also found […]

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Research scientists from Schering-Plough Research Institute developed a rapid high-performance liquid chromatography (HPLC) technique that allows for efficient separation and analysis of pharmaceutical compounds. The method uses a short column packed with 2.7μm “fused-core” silica particles that are made by fusing a 0.5μm layer of porous silica onto a solid silica particle[1]. These unique particles […]

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Today I studied my blog’s statistics to see what keywords people use to search in Google and Yahoo! to get to the pages of my site. Here is what I found based on the last 60 days of statistics: People used 1966 various search terms The top 20 keywords are: Then I looked at the […]

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As I mentioned in the last post from this series, capillary columns of 10–100 μm inner diameter are commonly used in nano-liquid chromatography setups. They are typically made either from fused silica or PEEK materials currently found in your everyday Gas Chromatography or HPLC, respectively. There are three basic types of capillary columns used in […]

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“HPLC for Pharmaceutical Scientists” is a new hardcover book edited by Yuri V. Kazakevich and Rosario LoBrutto with a price tag of $159 at Amazon. This is an excellent book for both novice and experienced pharmaceutical chemists that provides a unified approach to HPLC with a balanced treatment of the theory and practice of HPLC […]

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How many of you out there picked or even ate fresh bilberries? Probably not too many, unless you are from Northern Europe, Russia, or the Rocky Mountains of the US. The rest of us find bilberries in the form of purple capsules in dietary supplements. Bilberry is small shrub to a one foot in height […]

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Almost two weeks ago there was a question regarding Nano HPLC technology that I attempted to answer and in that post I mentioned Agilent as one of the pioneers in the Nano LC field. Agilent’s “nano” workhorse is a 1200 Series HPLC-Chip/MS system, which uses microfluidics chip-based technology for nanospray LC/MS. Based on the HPLC-Chip […]

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This is another Q&A from Yahoo! Answers which I could not stay away from. The question was asked by “pigpigletpoker” and has already received two answers. Dave_Stark said: Make a chromatogram of the paint found on the other car involved in the accident. Then do a side-by-side chromatogram of the red paint from the two […]

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Here is another example of forensic application of HPLC that allows to classify and date the black gel pen ink entries on documents. Gel pens first came out in 1984 in Japan and quickly became popular throughout the world due to their solid line, bold colors, and low cost. They are also regarded as environmentally […]

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