Articles on: Nano HPLC

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In theory, the analyte sensitivity increases on decreasing the column inner diameter.

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A review was published yesterday in “Journal of Separation Science” by a group of Italian researchers. So far all I have is abstract: Since its first introduction by Karlsson and Novotny in 1988 nano-LC has emerged as a complementary and/or competitive separation method to conventional HPLC, offering several advantages such as higher efficiency, ability to […]

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Scientists of the University of Twente (The Netherlands) succeed in speeding up the fluid flow in a micro channel using tiny and rapidly imploding gas bubbles that also provide a new way of mixing fluids within a lab-on-a-chip, without the need of complicated external components. The scientists led by Dr. Claus-Dieter Ohl of the MESA+ […]

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The HPLC method used a C8 column and the potassium chloride in aqueous acetic acid as a mobile phase with a linear gradient. The retention time was a bit over 20 min and the identity of the peak was confirmed as furosine by MS (mass spectrometry).

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Nano HPLC is a relatively new development in chromatography world driven by recent advancements in proteomics that would require decreasing of inner diameter (ID) of liquid chromatography (LC) column to allow for a smaller sample amount and to increase sensitivity.

  

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