Chromatography

Tags: adsorbent C18 ion exchange chromatography liquid liquid extraction lt 1 medical syringe mid 1990s packed column pipette tip solid phase extraction spe solid phase microextraction solid-phase extraction SPE SPME

Sample preparation is a crucial step in any analytical method, especially in chromatography where samples have to be homogenous, free of interferences and safe for the column. Today, one of the most widely used sample preparation methods is solid-phase extraction (SPE) that in the mid 1990s largely replaced the conventional but time consuming liquid-liquid extraction [...]

Tags: air particles airborne particulates atmospheric pollution cocaine cocaine dose column chromatography electrospray Gas Chromatography gas chromatography mass spectrometry GC/MS lc ms rome university solid-phase extraction soxhlet extraction

Cocaine is presently the most abused substance in Americas; however, the stimulant is gaining new advocates at an alarming rate in western Europe as well. In 2005, a group of researches from Italy analyzed water samples from the Po River in northern Italy for the presence of cocaine and its main urinary metabolite – benzoylecgonine [...]

Tags: CZE furosine g protein HPLC hplc method isoflavones linear gradient lysine residues Mass Spectrometry Nano HPLC potassium chloride powdered soy milk protein protein degradation solid-phase extraction solvent usage

The HPLC method used a C8 column and the potassium chloride in aqueous acetic acid as a mobile phase with a linear gradient. The retention time was a bit over 20 min and the identity of the peak was confirmed as furosine by MS (mass spectrometry).