Comments on: What is the response factor in chromatography?

By: parvatheesam

parvatheesam — Sat, 01 Oct 2011 04:40:53 +0000

you can use Anthracene also, but it is water insoluble. use acetonitrile to dissolve, do not mix even a drop of water as it undergo precipitation.

By: Parvatheesam

Parvatheesam — Sat, 01 Oct 2011 04:38:03 +0000

as it is primary standard and having specific absorbance

By: nehad

nehad — Tue, 06 Sep 2011 22:52:08 +0000

please send me reply

By: sondra

sondra — Mon, 07 Feb 2011 16:11:52 +0000

hi..

how do you identify triglycerides (by HPLC) using the fames analysis from gc??

thank you..

please reply

By: jagan

jagan — Sat, 20 Nov 2010 15:42:36 +0000

take 7.0buffer tablet n dissolve in 100ml of milli-Q water

By: nancy

nancy — Wed, 27 Oct 2010 07:04:45 +0000

Could anyone please help me in calculation for diluting internal standard for biodiesel analysis using GC-MS? should the concentration be in ppm? I am doing the enzymatic method for ethyl ester analysis. Please let me know around what range should be my internal standards?
thanks

By: shankar

shankar — Sun, 26 Sep 2010 10:25:44 +0000

how we prepare pH 7.0 buffer solutions?

By: AKSHAY MARVANIA

AKSHAY MARVANIA — Sat, 18 Sep 2010 17:43:02 +0000

HI EVERYBODY
I WANT TO KNOW THE CALCULATION OF HEIGHT OF PEAK.IF I HAVE THE DATE OF RELETIVE RESPONCE FACTOR,RELETIVE RETENTION TIME,% LIMIT.SO CAN I FIND THE HOW MANY PEAK WILL GIVE IN GHROMATOGRAM BY USING THIS VALUE.
THANK YOU

By: Karthek reddy

Karthek reddy — Wed, 15 Sep 2010 08:54:31 +0000

What is the Importance of RF in chromatography and difference between RF & RRF.

By: VSK

VSK — Tue, 03 Aug 2010 11:57:38 +0000

Hello Everybody,

Right now I know the RRF value of drug.
In quantitative calculation wheather i have to multiply or divide?

By: Rohini.k

Rohini.k — Tue, 15 Jun 2010 15:41:29 +0000

WHY WE USE RF VALUES FROM 0.2 TO 5.0

By: Rohini.k

Rohini.k — Tue, 15 Jun 2010 15:38:21 +0000

1. Definition of relative response factor :
A measure of the relative response of the instrument detector to an analyte compared to an internal or external standard. Relative Response Factors are determined by the analysis of standards and are used to calculate the concentrations of analytes in samples. The RRF can be calculated using the following equation:

RFF = (Ac x Cis)/(Ais x Cc)

where,
RRF = Relative response factor
Ac = Area of the target analyze
Ais = Area of the corresponding internal standard
Cis = Concentration of the corresponding internal standard
Cc = Concentration of the target analyte

By: Rohini.k

Rohini.k — Tue, 15 Jun 2010 15:37:35 +0000

Dear, All

If u want better clarification on RF & RRF read once B.P

By: Rohini.k

Rohini.k — Tue, 15 Jun 2010 15:36:47 +0000

Dear, All

If want better clarification on RF & RRF read once B.P

By: surya 4m hyd

surya 4m hyd — Thu, 03 Jun 2010 04:12:30 +0000

dear sir, I want to know actual response of a known impurity in my sample. is there any relation between RRF & area percent of that impurity obtained in the sample?

By: kiran reddy

kiran reddy — Fri, 28 May 2010 10:34:09 +0000

caffiene,
it gives multi wavelength responce like 205,245,273 nm
it gives two maxima and one minima wave lengths.

By: Najm

Najm — Tue, 04 May 2010 01:47:01 +0000

How do you calculate peak area when given
time
Height (mAU)
Area (mAU.min)
Area %

In relation to HPLC
thanks

By: danger

danger — Mon, 26 Apr 2010 18:24:01 +0000

pl.dont calculate response facotr.dont take any type of tension.cool & enjoy urself.

By: m.r

m.r — Thu, 22 Apr 2010 03:55:21 +0000

Dear sir,
please help me.
I want quntitive of response factor 1,3 butadiene relative of vinyl actylene in gc .
for example if RF 1,3 BD 4.2
Vinyl actylene (1-butene-3-yne)= ???
thanks

By: elle

elle — Sat, 27 Mar 2010 12:48:26 +0000

Dear sir,

I’ve been doing a research about caffeine in certain brands of coffee using GC/MSD. The problem is that i don’t know how to calculate the amount of caffeine using the area that obtained from GC/MSD. How am i going to calculate the amount of caffeine?? What information should i have? And what formula should i use? Please help me. Please3…

By: GOPI

GOPI — Sun, 07 Mar 2010 20:11:02 +0000

why use caffiene for hplc calibration

By: rohini.k

rohini.k — Thu, 18 Feb 2010 08:54:54 +0000

We calculate RF in calculation sheet as multify the given RF Value.

By: kishore

kishore — Sun, 14 Feb 2010 15:44:14 +0000

Respcted sir

I want to know about response factor and validation of analytical methods calculations.

By: mojtab

mojtab — Thu, 04 Feb 2010 14:50:58 +0000

please help me.
i want calculation of response factor in gc .
thanks

By: amol

amol — Sat, 30 Jan 2010 10:30:53 +0000

how i calculate response factor in hplc ?

By: amol

amol — Sat, 30 Jan 2010 10:30:03 +0000

how i calculate response factor in HPLC

By: mystmonkey

mystmonkey — Wed, 13 Jan 2010 08:59:06 +0000

Internal standard how much do i put and why , how do i choose the internal standard . I am working on identifying the volatile constituents of some fruits and their quantities using GC and HPLC . how do i kno which internal standard and what quantities of it should i use .