During my frequent trips to south-east Asia, I always enjoy a very peculiar, tropical fruit - mangosteen. It is one of the most praised of tropical fruits, and certainly the most esteemed fruit in the family Guttifera. The fruit is round and about 1.5 -2″ in diameter with a very dark purple smooth skin. It is better to open it with a knife by cutting around the middle completely through the rind, and lifting off the top half, which leaves the snow-white, juicy, soft segments exposed in the colorful “cup”??the bottom half of the rind. The fruit is exquisitely luscious and delicious, and it is by far my most favorite taste of all.
The rind of partially ripe fruits yields a polyhydroxy-xanthone also called ?-mangostin; whereas, fully ripe fruits contains the xanthones and various derivatives. Xanthones are unique chemical compounds composed of a tricyclic aromatic system with a variety of phenolic, methoxy, and isoprene substituents, giving rise to numerous derivatives. They dissolve to varying degrees in solvents ranging from alcohol to hexane.
Scientist at Weber State University developed methods for extraction, identification and quantitation of xanthones and their derivatives: alpha-mangostin, 8-desoxygartanin, gartanin, beta-mangostin, 3-mangostin, and 9-hydroxycalabaxanthone. They found that the optimum solvent mixture of acetone/water (80:20) selectively and effectively extracts a wide variety of xanthones and subsequent [tag]HPLC[/tag] analysis using standard C 18 Reverse Phase column and a 30-min gradient of 65-90% methanol in 0.1% formic acid detects and separates numerous different xanthones with UV detection at 254 nm.
Tags: aromatic_system HPLC mangosteen weber_state_university xanthones
6 Responses
Zulu
April 28th, 2007 at 7:04 pm
1I’ve seen this fruit in market when I was in Thailand but never got around to try it. Now after your review I might have to next time I go.
Ice Mah
January 14th, 2008 at 10:35 pm
2I’m very interested in your HPLC analysis of xanthone in mangosteen. Can I ask you some questions?
1. What is the extraction process and crude ectract process? includes the solvents and methods.
2. what is the purification process?
3. What types of adsorbent you are using?
What is the suitable adsorbent for purificanion of phenolic compounds in mangosteen?
4. What is the mobile phase you are using? and the sequence of solvent being used?
5. What is the physical indicator to justify that elution proces is completed?
6. Im your opinion, what is the suitable type of elution solvent commonly used ?
7. normally, eluants are in mass or volume form?\
Hope to hear from you soon. Thank you for you attention. you can directly reply to my e-mail icexxm@hotmail.com.
Julie Tan
April 8th, 2008 at 4:32 am
3in the absence of HPLC how can i isolate and extract xnathone, even though crude xanthone only.
Chews4Health Leaders
August 24th, 2008 at 5:33 pm
4Fruits and berries offer tremendous health enhancing qualities and contain hundreds of naturally occurring vitamins, minerals, antioxidants, amino acids, enzymes, essential fatty acids, fiber & phytonutrients. The 4 ??Super Fruits? contained in Chews-4-Health are considered by many experts to be the most nutrient rich fruits the world has to offer. Our carefully selected super-fruit complex gives you the power of all 4 in one supplement.
randoft
September 29th, 2008 at 12:45 pm
5My final year project is almost similar with your title here…extraction of xanthone and analyze using GC-MS….not HPLC…i wan to ask u some questions:
!) can it be using GC-MS to analyze xanthone?
2) May i know what is the extraction process and crude ectract process? includes the solvents and methods.
3) what is the m/z ratio of xanthone in MS?
4) The extraction method of xanthone for another fruit is it similar to mangosteen?
cecile cabanag
August 3rd, 2010 at 11:30 am
6hi. why do you consider acetone:water (80:20) as the best solvent for mangosteen periicarp extraction? why is it better than ethanol? we are planning to test the pericarp’s extract against a certain bacteria, how can we purify further the extract? we dont have plans to use chromatography in this experiment. thank you
RSS feed for comments on this post · TrackBack URI
Leave a reply
Random Posts
Categories
Tag Cloud
adsorbent adsorption benzene china chlorophyll chromatographic chromatography column_chromatography CZE DESI electromagnetic_induction electrospray ELISA eluent explosives forensic furosine Gas Chromatography GC/MS History HPLC IC isoflavones lab-on-a-chip LCMS liquid_chromatography maldi_tof Mass Spectrometry microfluidic nano nanogenerator Nano HPLC nanoliquid nanoscale nanostream nanotechnology nanotubes News organic_molecule protein russian_chemist science soy TCM tsvet
Feed Tools
Recent Posts
Chemistry Resources
Health
Recent Entries
Recent Comments
Most Commented
All Rights Reserved 2007 | Chromatography