Nano LC Sensitivity
Posted by Chemist as Nano HPLC on Aug 05, 2007
(1 votes, average: 4 out of 5)
Loading ...Nano Liquid Chromatography
In theory, the analyte sensitivity increases on decreasing the column inner diameter; however, because of the very low sample volumes injected - 20-60 nL, nano liquid chromatography cannot be considered as an analytical method of high sensitivity.
There are several research studies that focused on the improvement of sensitivity by using techniques such as on-column focusing[1] and 2-D separation[2].
1. “Trace Level Determination of Organophosphorus Pesticides in Water with the New Direct-Electron Ionization LC/MS Interface”, Achille Cappiello et al., Analytical Chemistry, 2002 vol. 74 pp. 3547-54
2. “Fully automated micro- and nanoscale one- or two-dimensional high-performance liquid chromatography system for liquid chromatography-mass spectrometry compatible with non-volatile salts for ion exchange chromatography” by Masuda, J. et al., Journal of Chromatography A 2005, 1063, pp. 57â??69.
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3 Responses
Ms. Buckyball
August 6th, 2007 at 5:15 am
1Hi!
I am interested on chiral molecules and ways to separate them. Is it possible to discuss chiral chromatography? Thanks!
Chemist
August 6th, 2007 at 6:29 am
2chiral chromatography? The only time I did enantiomer separation was when I worked at Merck. I remember we used polysaccharide based stationary phase and I hated it so much because nothing ever worked.
However, it is a very good topic! I think I am going to do a review of CC principles and methods, or maybe you have something else in mind?
Ms. Buckyball
August 8th, 2007 at 5:55 am
3I also think it is a good topic to discuss here on your blog. In my organic chemistry, there are many ways to separate enantiomers. But this topic is really an interesting one. It shows how diverse Chemistry is! I hope, you could able to discuss its principles, method, application and lastly, any news and articles that pertain to CC.
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